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KMID : 0903519990420020123
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1999 Volume.42 No. 2 p.123 ~ p.127
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Isolation and Identification of Genes Regulated by Iron Using mRNA Differential Display
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Abstract
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Iron is an essential nutrient but potentially toxic element in human. To identify the effects of iron on the gene expression of mammalian cell, we have isolated several genes that are regulated by iron using the RNA differential display method. RNAs were isolated from HeLa cells treated with iron supplement or iron chelator. A total of 24 genes were isolated and of these, four genes were identified by DNA sequencing and northern blot.
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